Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of ERα gene ( A ) and ERα protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using t -test *, p < 0.005.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERα protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of the ERβ gene ( A ) and ERβ protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using a t -test * p < 0.05.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERβ protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: p53 status of each GBM cell line.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Mutagenesis

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Diverse expression of cell death-related genes by TTFields in GBM cell lines (U87, U251, U373, and T98G) following TTFields treatment. ( a ) Venn diagram of cell death-related genes by TTFields in four indicated GBM cell lines. The groups were divided by TP53 status into the WT (U87) and MT (U251, U373, T98G) TP53 groups. ( b ) Expression of common genes in cell death after TTFields treatment represented by clustering analysis in WT and MT TP53 cells. ( c ) Overlapping MT TP53 cells (U251, U373, and T98G). ( d ) Heat map showing the effects of altered genes by TTFields on cell death in MT TP53 cells. Increased and decreased gene expression is indicated in red and blue colours, respectively. All data represent > 1.5-fold changed genes.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Expressing, Gene Expression

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Differential expression of cell cycle-related genes by TTFields in GBM cell lines. ( a ) Venn diagram of cell cycle-related genes by TTFields in GBM cell lines. ( b ) Expression of common genes related to cell cycle by TTFields in GBM cells. ( c ) Overlapping MT TP53 cells (U251, U373, and T98G). ( d ) Heat map showing cell cycle-related genes with > 1.5-fold change in expression following TTFields treatment in MT TP53 cells. Each spot indicates altered gene after TTFields treatment in the cells. Intensity is represented in red (increased) and blue (decreased) colours. All data represent ≥ 1.5-fold change in gene expression.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Quantitative Proteomics, Expressing, Gene Expression

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Gene expression profiling of GBM cell lines by TTFields on immune and inflammatory responses. ( a ) Venn diagram of immune and inflammatory response-related genes altered by TTFields treatment in GBM cell lines. ( b ) Expression of common genes involved in immune and inflammatory responses in GBM cells following TTFields treatment, as indicated by clustering analysis. ( c ) Overlapping results in MT TP53 cells (U251, U373, and T98G). ( d ) Heat map presents immune and inflammatory response-related genes showing ≥ 1.5-fold change in expression following TTFields treatment in MT TP53 cells. Intensity is indicated by red (increased) and blue (decreased). All data represent ≥ 1.5-fold change in gene expression.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Validation of gene expression in microarray data by qRT-PCR. ( a ) Co-expression of mRNAs of identified genes and TP53 in patients with GBM. ( b – d ) qRT-PCR results for GPNMB, KRT19, and KRT15 expressed as log2 gene expression changes (ΔΔCt). qRT-PCR data are presented as the mean ± standard deviation (n = 3).

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Biomarker Discovery, Gene Expression, Microarray, Quantitative RT-PCR, Expressing, Standard Deviation

Journal: Nature Communications

Article Title: Late-stage (radio)fluorination of alkyl phosphonates via electrophilic activation

doi: 10.1038/s41467-024-54208-y

Figure Lengend Snippet: a Late-stage radiofluorination of PET tracers and 18 F-synthons. RCC TLC determined by radio-TLC ( n = 3); RCY = isolated 18 F-product activity amount/starting amount of radioactivity (decay corrected). cLog P values were predicted using ALOGPS 2.1 ( http://www.vcclab.org/lab/alogps ). Blue arrows pointing upward indicate an increase in cLog P /Log D 7.4 compared to the parent compound, while downward arrows indicate a decrease. The small pink ball after cLog P /Log D 7.4 represents the target of the parent compound. b Preparation α v β 3 integrin receptor developer [ 18 F]BFPA-E[c(RGDyK)] 2 . c MicroPET images of [ 18 F]BFPA-E[c(RGDyK)] 2 in U87MG xenograft mice at 30 min after tail vein injection. 200 μg of E[c(RGDyK)] 2 was used to block the tumor uptake of [ 18 F]BFPA-E[c(RGDyK)] 2 . The white circle is the tumor area. d Preparation of PET tracer [ 18 F]BFPA-Flurpiridaz targeting MC I. e MicroPET/CT images of healthy mice [ 18 F]BFPA-Flurpiridaz 60 min after caudal vein injection. f Molecular Docking. Flurpiridaz and BFPA-Flurpiridaz to MC I (PDB: 7ZM8). Cyan: residues composing the substrate-binding cavity; yellow: residues forming hydrogen bonds; yellow dashed lines: locations of hydrogen bond.

Article Snippet: The glioma cell line U87MG was obtained from the China Center for Type Culture Collection of the Chinese Academy of Sciences.

Techniques: Isolation, Activity Assay, Radioactivity, Injection, Blocking Assay, Binding Assay

Journal: Scientific Reports

Article Title: HSV1 microRNAs in glioblastoma development: an in silico study

doi: 10.1038/s41598-023-45249-2

Figure Lengend Snippet: The volcano plots of the included samples from the GSE158284 and GSE13276 datasets and the common genes between DEGs and targets of hsv1-miR-H6-3p and hsv1-miR-H1-5p. ( A ) The differentially expressed genes (DEGs) of included samples from the GSE158284 dataset with the cut-off of adjusted P-value ˂ 0.05 and |log2FC| ≥ 1.3. ( B ) The DEGs of the included samples from the GSE158284 dataset adjusted P-value ˂ 0.05 and |log2FC| ≥ 1.3. (C) The common genes between DEGs and targets of hsv1-miR-H6-3p and hsv1-miR-H1-5p. ( C ) The common genes between targets of hsv1-miR-H6-3p and the downregulated genes in GBM tissues. ( D ) The common genes between targets of hsv1-miR-H1-5p and the upregulated genes in GBM tissues.

Article Snippet: The Human Protein Atlas ( https://www.proteinatlas.org/ ) was used to identify the protein localization of DEGs in human GBM cell lines .

Techniques: